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DNA fragmentation index test

The integrity of genetic material in the sperm is critical for successful fertilization and normal embryos development. Usually, male infertility is caused by the poor quality or quantity of sperm, either the sperm produced by the male has a low sperm count or it's of low quality. Unfortunately in semen analysis test the count,motility and morphology is analysed but functionality of sperm can’t be assessed whether it will be able to undergo the changes required at the time of fertilisation or not.

Infertility specialist in Lucknow

DNA, a deoxyribonucleic acid, is present in all living cells and it carries the genetic information.DNA fragmentation refers to the problems in the genetic composition of the sperm which if high leads to infertility in Male or be a reason for a failed pregnancy many times .The sperm DNA fragmentation test accurately identify men who have high or low fragmentation index which tells us about the functionality of sperm and many times with high DFI they are less likely to achieve a full-term pregnancy either naturally or through Assisted Reproductive Technologies. The best Lucknow IVF centre is well known to conduct the DNA fragmentation test in males who have such problems.

Infertility specialist in Lucknow

Causes of DNA fragmentation, but there is no solid proof backing what exactly causes it:

  • • Obesity
  • • Ageing
  • • Unhealthy lifestyle
  • • Unhealthy eating habit
  • • Unhealthy environment
  • • Smoking
  • • High fever
  • • stress
  • • alcohol consumption.
  • • infection
  • • pyrexia
  • • elevated testicular temperature
  • • recreational drugs
  • • sexual disease
  • • varicocele
  • • elevated testicular temperature
  • Effects of DNA Fragmentation

  • • The higher the levels of DNA fragmentation, the higher the chances of failed pregnancy
  • • abnormal sperm parameters are high in subfertilemen with high DNA fragmentation
  • • it affects the blastocyst development by apoptosis and mutation leading to miscarriage and abnormalities in the offspring
  • • poor prognosis embryo that had high DNA fragmentation
  • Importance of DNA fragmentation

    DNA fragmentation test is important as it can help the doctors to diagnose, know and manage male infertility and find out men who are at risk of subfertility.it provides a prognostic valve in assessing the outcome of assisted conception treatment Infertility specialist in Lucknow has an excellent way to resolve and treat the problem of male infertility.

    Specific tests designed for DNA fragmentation

  • • SCSA
  • Sperm chromatin structure assay test gives information about the genetic quality of the sperm helping in male infertility.it is an advanced male infertility test

    Primary factors messed by SCSA are:

  • • % DFI ( DNA fragmentation index)
  • Reports on the percentage of sperm that have DNA fragmentation

  • • %HDS( High DNA Stainability)
  • • Reports on the percentage of sperm with abnormal nuclear protein and chromatin structure that may inhibit fertilization
  • • TUNEL
  • Terminal deoxynucleotidyl transferase dUTP nick end labelling is a highly sensitive and reproducible method of assessing the integrity of sperm

    It is a method for detecting DNA fragmentation by labelling the 3'- hydroxyl termini in the double-strand

    This test can be offered to infertile patients who are idiopathic, have severe oxidative stress-related abnormal semen quality visit the best infertility doctor in Lucknow for the solution

  • • SpermComet Test
  • In this test how much damage DNA is damaged in each sperm is known .it is a second-generation sperm DNA test

    Sperm are stained with a fluorescent dye that interacts with the DNA molecule. the fluorescence signal changes when the DNA is fragmented and by using a cytometer it is monitored.

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    When one should get DFI test done ?

    If you are suffering from -

  • • Unexplained infertility
  • • Multiple ivf failures
  • • Recurrent abortion’s
  • • Recurrent pregnancy loses
  • • Severe OATS –oligoasthenoteratozoospermia